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EGFR (phospho Tyr1173) antibody [9H2]

Anti-EGFR (phospho Tyr1173) antibody [9H2] used in Western Blot (WB). GTX42900

Cat. No. GTX42900

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9H2

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
50 μg ($519)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1173 of mature EGFR is equivalent to Tyr1197 of uncleaved EGFR.

PROPERTIES

Form

Liquid

Buffer

PBS, PEG, Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane , Endoplasmic reticulum membrane , Endosome , Endosome membrane , Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Tyr1173) antibody [9H2] used in Western Blot (WB). GTX42900

GTX42900 WB Image

WB analysis of mock control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 cell lysate using GTX42900 EGFR (phospho Tyr1173) antibody [9H2].
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519