IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies ,GTX80162 (A), GTX80161 (B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with GTX80162 EGFR (phospho Tyr992) antibody [EM-12] or GTX80161 EGFR (phospho Tyr1173) antibody [EM-13] antibody.

IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies GTX80162(A), GTX80161(B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal antibody.

WB analysis of EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines using GTX80161 EGFR (phospho Tyr1173) antibody [EM-13].