APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1173 of mature EGFR is equivalent to Tyr1197 of uncleaved EGFR.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EGFR , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , epidermal growth factor receptor , mENA , EGF Receptor
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161")
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GTX80161 WB Image
WB analysis of EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines using GTX80161 EGFR (phospho Tyr1173) antibody [EM-13].
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GTX80161 IP Image
IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies GTX80162(A), GTX80161(B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal antibody.
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GTX80161 IP Image
IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies ,GTX80162 (A), GTX80161 (B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with GTX80162 EGFR (phospho Tyr992) antibody [EM-12] or GTX80161 EGFR (phospho Tyr1173) antibody [EM-13] antibody.
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REFERENCE
There are currently no references for EGFR (phospho Tyr1173) antibody [EM-13] (GTX80161). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1173) antibody [EM-13] (GTX80161). Be the first to share your experience with this product.
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161](/upload/website/prouct_img/small/GTX80161/GTX80161_20191025_AP_001_402_w_23061322_170.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161")
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161](/upload/website/prouct_img/small/GTX80161/GTX80161_20191025_AP_007_401_w_23061322_407.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161")
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161](/upload/website/prouct_img/small/GTX80161/GTX80161_20191025_AP_007_400_w_23061322_119.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161")
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161](/upload/website/prouct_img/normal/GTX80161/GTX80161_20191025_AP_001_402_w_23061322_170.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Western Blot (WB). GTX80161")
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161](/upload/website/prouct_img/normal/GTX80161/GTX80161_20191025_AP_007_401_w_23061322_407.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161")
![Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161](/upload/website/prouct_img/normal/GTX80161/GTX80161_20191025_AP_007_400_w_23061322_119.webp "Anti-EGFR (phospho Tyr1173) antibody [EM-13] used in Immunoprecipitation (IP). GTX80161")