APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 (starvation for 16 hr and 100ng/ml EGF treatment for 5 min)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.91 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1173 of human EGFR. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888476
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
![Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793")
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GTX634793 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1173) antibody [GT1111] (GTX634793) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for EGFR (phospho Tyr1173) antibody [GT1111] (GTX634793). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1173) antibody [GT1111] (GTX634793). Be the first to share your experience with this product.
![Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793](/upload/website/prouct_img/small/GTX634793/GTX634793_43346_20181026_WB_treatment_EGF_19011519_652.jpg "Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793")
![Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793](/upload/website/prouct_img/normal/GTX634793/GTX634793_43346_20181026_WB_treatment_EGF_19011519_652.jpg "Anti-EGFR (phospho Tyr1173) antibody [GT1111] used in Western Blot (WB). GTX634793")