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EGFR (phospho Tyr1173) antibody

Orthogonal
Anti-EGFR (phospho Tyr1173) antibody used in IHC (Paraffin sections) (IHC-P). GTX25652
Anti-EGFR (phospho Tyr1173) antibody used in IHC (Paraffin sections) (IHC-P). GTX25652
Anti-EGFR (phospho Tyr1173) antibody used in ELISA (ELISA). GTX25652
Anti-EGFR (phospho Tyr1173) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25652

Cat. No. GTX25652

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:500
IHC-P 1:10-1:100
FACS 1:20
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1173 of mature EGFR is equivalent to Tyr1197 of uncleaved EGFR.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1173. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Tyr1173) antibody used in IHC (Paraffin sections) (IHC-P). GTX25652

GTX25652 IHC-P Image

IHC-P analysis of human lung adenocarcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

Anti-EGFR (phospho Tyr1173) antibody used in IHC (Paraffin sections) (IHC-P). GTX25652

GTX25652 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-EGFR (phospho Tyr1173) antibody used in ELISA (ELISA). GTX25652

GTX25652 ELISA Image

ELISA analysis of phopsho-EGFR(Tyr1173) using GTX25652 EGFR (phospho Tyr1173) antibody. The antibody was blocked with different phospho-EGFR peptides.

Anti-EGFR (phospho Tyr1173) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25652

GTX25652 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with EGF (200 ng/mL for 10 min) using GTX25652 EGFR (phospho Tyr1173) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399