ICC/IF analysis of HeLa cells treated with EGF (200 ng/mL for 10 min) using GTX25652 EGFR (phospho Tyr1173) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

ELISA analysis of phopsho-EGFR(Tyr1173) using GTX25652 EGFR (phospho Tyr1173) antibody. The antibody was blocked with different phospho-EGFR peptides.

IHC-P analysis of human hepatocellular carcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

IHC-P analysis of human lung adenocarcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50