APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1:100-1:500 |
| 1:10-1:100 |
| 1:20 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1173 of mature EGFR is equivalent to Tyr1197 of uncleaved EGFR.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 1mg/ml BSA, 50% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1173. The sequence is conserved in mouse and rat.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX25652 IHC-P Image
IHC-P analysis of human hepatocellular carcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX25652 ICC/IF Image
ICC/IF analysis of HeLa cells treated with EGF (200 ng/mL for 10 min) using GTX25652 EGFR (phospho Tyr1173) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
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GTX25652 IHC-P Image
IHC-P analysis of human lung adenocarcinoma tissue using GTX25652 EGFR (phospho Tyr1173) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50
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GTX25652 ELISA Image
ELISA analysis of phopsho-EGFR(Tyr1173) using GTX25652 EGFR (phospho Tyr1173) antibody. The antibody was blocked with different phospho-EGFR peptides.
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REFERENCE
There are currently no references for EGFR (phospho Tyr1173) antibody (GTX25652). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1173) antibody (GTX25652). Be the first to share your experience with this product.
