Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR (phospho Tyr845) antibody [HL3331] (GTX641096) diluted at 1:20000 and competitor's antibody (Highly Cited Competitor) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

EGFR (phospho Tyr845) antibody [HL3331] detects EGFR (phospho Tyr845) protein by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EGFR (phospho Tyr845) stained by EGFR (phospho Tyr845) antibody [HL3331] (GTX641096) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr845) antibody [HL3331] (GTX641096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.