Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EGFR (phospho Tyr845) antibody detects EGFR (phospho Tyr845) protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EGFR (phospho Tyr845) antibody detects EGFR (phospho Tyr845) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr845) protein stained by EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:500.
Blue: Hoechst 33342 staining.