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EGR1 antibody, N-term

Anti-EGR1 antibody, N-term used in Western Blot (WB). GTX81956
Anti-EGR1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81956
Anti-EGR1 antibody, N-term used in Western Blot (WB). GTX81956
Anti-EGR1 antibody, N-term used in Flow cytometry (FACS). GTX81956

Cat. No. GTX81956

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($399)
See all EGR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Predict Reactivity

Bovine, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human EGR1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11173709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

early growth response 1 , AT225 , G0S30 , KROX-24 , NGFI-A , TIS8 , ZIF-268 , ZNF225

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-EGR1 antibody, N-term used in Western Blot (WB). GTX81956

GTX81956 WB Image

WB analysis of NIH-3T3 tissue lysate (35ug/lane) using GTX81956 EGR1 antibody, N-term.

Anti-EGR1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81956

GTX81956 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81956 EGR1 antibody, N-term.
Green : EGR1
Blue : DAPI

Anti-EGR1 antibody, N-term used in Western Blot (WB). GTX81956

GTX81956 WB Image

WB analysis of MDA-MB231 cell lysate (35ug/lane) using GTX81956 EGR1 antibody, N-term.

Anti-EGR1 antibody, N-term used in Flow cytometry (FACS). GTX81956

GTX81956 FACS Image

FACS analysis of WiDr cells using GTX81956 EGR1 antibody, N-term.
Green : primary antibody
Blue : negative control

REFERENCE

Application Reference

Hou J et al. Behav Brain Res 2019; 364 : 167-176 rem sleep deprivation-induced circadian clock gene abnormalities participate in hippocampal-dependent memory impairment by enhancing inflammation in rats undergoing * inhalation.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399