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EGR1 antibody

Overexpression
Anti-EGR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129015
Anti-EGR1 antibody used in Western Blot (WB). GTX129015
Anti-EGR1 antibody used in Western Blot (WB). GTX129015

Cat. No. GTX129015

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EGR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EGR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885870

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

early growth response 1 , AT225 , G0S30 , KROX-24 , NGFI-A , TIS8 , ZIF-268 , ZNF225

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129015

GTX129015 ICC/IF Image

EGR1 antibody detects EGR1 protein at cytoplasm by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGR1 stained by EGR1 antibody (GTX129015) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EGR1 antibody used in Western Blot (WB). GTX129015

GTX129015 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EGR1 antibody (GTX129015) diluted at 1:5000.

Anti-EGR1 antibody used in Western Blot (WB). GTX129015

GTX129015 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EGR1 antibody (GTX129015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 24098453

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169