APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
EGR1-transfected 293T cells
Predict Reactivity
Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human EGR1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
early growth response 1 , AT225 , G0S30 , KROX-24 , NGFI-A , TIS8 , ZIF-268 , ZNF225
Cellular Localization
Nucleus
Background
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129015 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EGR1 antibody (GTX129015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 24098453
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GTX129015 WB Image
EGR1 antibody detects EGR1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human EGR1-transfected 293T cells
10 % SDS-PAGE
EGR1 antibody (GTX129015) dilution: 1:5000
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REFERENCE
There are currently no references for EGR1 antibody (GTX129015). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGR1 antibody (GTX129015). Be the first to share your experience with this product.
![Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214](/upload/website/prouct_img/small/GTX70214/GTX70214_41848_20160623_WB_shRNA_watermark_18121411_112.jpg "Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214")
