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EGR2 antibody

Anti-EGR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102912
Anti-EGR2 antibody used in Western Blot (WB). GTX102912
Anti-EGR2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102912
Anti-EGR2 antibody used in Western Blot (WB). GTX102912

Cat. No. GTX102912

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all EGR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

EGR2 transfected 293T

Predict Reactivity

Rat, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EGR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950178

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

early growth response 2 , AT591 , CHN1 , CMT1D , CMT4E , KROX20

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102912

GTX102912 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EGR2 antibody (GTX102912) at 1:300 dilution.

Anti-EGR2 antibody used in Western Blot (WB). GTX102912

GTX102912 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
10% SDS PAGE
GTX102912 diluted at 1:1000

Anti-EGR2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102912

GTX102912 IHC-Fr Image

Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using EGR2(GTX102912) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGR2 antibody used in Western Blot (WB). GTX102912

GTX102912 WB Image

Western Blot analysis of EGR2 expression in transfected 293T cell line by EGR2 polyclonal antibody.
A: Non-transfected lysate.
B: EGR2 transfected lysate.
12% SDS PAGE
GTX102912 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kuramoto M et al. Int Endod J 2020; (Epub) Mineral trioxide aggregate suppresses pro-inflammatory cytokine expression via the calcineurin/nuclear factor of activated T cells/early growth response 2 pathway in lipopolysaccharide-stimulated macrophages.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169