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EGR3 antibody

Comparison Orthogonal
Anti-EGR3 antibody used in Western Blot (WB). GTX129199
Anti-EGR3 antibody used in Western Blot (WB). GTX129199

Cat No. GTX129199

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all EGR3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

mouse brain , rat brain , MCF-7 , MCF-7 nuclear extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EGR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

early growth response 3 , EGR-3 , PILOT

Cellular Localization

Nucleus

Background

The gene encodes a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins. It is an immediate-early growth response gene which is induced by mitogenic stimulation. The protein encoded by this gene participates in the transcriptional regulation of genes in controling biological rhythm. It may also plays a role in muscle development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGR3 antibody used in Western Blot (WB). GTX129199

GTX129199 WB Image

Comparison Orthogonal

MCF-7 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGR3 antibody (GTX129199) diluted at 1:500 and competitor's antibody (sc-191) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGR3 antibody used in Western Blot (WB). GTX129199

GTX129199 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGR3 antibody (GTX129199) diluted at 1:300. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169