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EHHADH antibody, C-term

Anti-EHHADH antibody, C-term used in Western Blot (WB). GTX81126
Anti-EHHADH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81126
Anti-EHHADH antibody, C-term used in Flow cytometry (FACS). GTX81126
Anti-EHHADH antibody, C-term used in Western Blot (WB). GTX81126

Cat. No. GTX81126

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($399)
See all EHHADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11163333

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase , ECHD , FRTS3 , L-PBE , LBFP , LBP , PBFE

Background

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-EHHADH antibody, C-term used in Western Blot (WB). GTX81126

GTX81126 WB Image

WB analysis of mouse kidney, rat kidney and liver tissue using GTX81126 EHHADH antibody, C-term.
Loading : 35ug per lane
Dilution : 1:1000

Anti-EHHADH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81126

GTX81126 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81126 EHHADH antibody, C-term.

Anti-EHHADH antibody, C-term used in Flow cytometry (FACS). GTX81126

GTX81126 FACS Image

FACS analysis of HepG2 cells using GTX81126 EHHADH antibody, C-term.
Top histogram : negative control
Bottom histogram : HepG2 cells

Anti-EHHADH antibody, C-term used in Western Blot (WB). GTX81126

GTX81126 WB Image

WB analysis of mouse liver(lane 1), kidney(lane 2) tissue lysate (35ug/lane) using GTX81126 EHHADH antibody, C-term.

REFERENCE

Application Reference

Ranea-Robles P et al. J Inherit Metab Dis 2021; 44 (6) : 1419-1433 The peroxisomal transporter ABCD3 plays a major role in hepatic dicarboxylic fatty acid metabolism and lipid homeostasis.
Application : WB, IHC-P
Reactivity : Mouse
Pablo Ranea-Robles et al. Cell Mol Life Sci 2021; 78 (14) : 5631-5646 Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.
Application : WB
Reactivity : Human
Pablo Ranea-Robles et al. Kidney360 2021; 2 (9) : 1441-1454 Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.
Application : WB, IHC-P
Reactivity : Mouse
Violante S et al. FASEB J 2018; : fj201801498R Peroxisomes can oxidize medium- and long-chain fatty acids through a pathway involving ABCD3 and HSD17B4.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399