GeneTex
United States (US)

EHHADH antibody

EHHADH-antibody-GTX87989-WB-1_18121410_563.jpg
EHHADH-antibody-GTX87989-IHC-1_18121410_635.jpg

Cat No. GTX87989

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

79 kDa. ( Note )

Specificity/Sensitivity

EHHADH Antibody detects endogenous levels of total EHHADH protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human EHHADH.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enoyl-Coa Hydratase And 3-Hydroxyacyl Coa Dehydrogenase,Echd,Frts3,L-Pbe,Lbfp,Lbp,Pbfe,Ehhadh

Background

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
EHHADH-antibody-GTX87989-WB-1_18121410_563.jpg

GTX87989 WB Image

Western blot analysis of extracts from A549 cells, using EHHADH Antibody. The lane on the right is treated with the synthesized peptide.

EHHADH-antibody-GTX87989-IHC-1_18121410_635.jpg

GTX87989 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EHHADH Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339