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EIF1A antibody

Anti-EIF1A antibody used in Western Blot (WB). GTX118482
Anti-EIF1A antibody used in IHC (Paraffin sections) (IHC-P). GTX118482
Anti-EIF1A antibody used in Western Blot (WB). GTX118482
Anti-EIF1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118482

Cat. No. GTX118482

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Raji , PC-12

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF1AY. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630070

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 1A Y-linked , eIF-4C

Background

This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF1A antibody used in Western Blot (WB). GTX118482

GTX118482 WB Image

EIF1AY antibody detects EIF1AY protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
15 % SDS-PAGE
EIF1AY antibody (GTX118482) dilution: 1:1000

Anti-EIF1A antibody used in IHC (Paraffin sections) (IHC-P). GTX118482

GTX118482 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using EIF1AY(GTX118482) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF1A antibody used in Western Blot (WB). GTX118482

GTX118482 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX118482 diluted at 1:500

Anti-EIF1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118482

GTX118482 ICC/IF Image

EIF1AY antibody detects EIF1AY protein at Golgi apparatus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold Methanol for 5 min.
Green: EIF1AY protein stained by EIF1AY antibody (GTX118482) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169