GeneTex
United States (US)

EIF2 beta antibody

KO/KD
GTX106484_40135_WB_R_19011506_274.jpg
GTX106484_WB_19011506_399.jpg
GTX106484_40135_IHC_19011506_668.jpg
GTX106484_40135_IFA_19011506_698.jpg
GTX106484_40135_20150529_IHC_M_19011506_142.jpg
GTX106484_40135_WB_M_liver_19011506_786.jpg

Cat No. GTX106484

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , mouse liver , PC-12 , Rat2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686L18198 antibody, EIF2 antibody, EIF2B antibody, EIF2beta antibody, MGC8508 antibody, EIF2S2 antibody, eukaryotic translation initiation factor 2 subunit beta antibody, eIF-2-beta antibody, protein phosphatase 1, regulatory subunit 67 antibody, eukaryotic initiation factor 2-beta antibody, eukaryotic translation initiation factor 2 beta antibody, eukaryotic translation initiation factor 2 subunit 2 antibody, eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody

Background

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]
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Database

DATA IMAGES
GTX106484_40135_WB_R_19011506_274.jpg

GTX106484 WB Image

EIF2 beta antibody detects EIF2S2 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
EIF2 beta antibody (GTX106484) dilution: 1:2000

GTX106484_WB_19011506_399.jpg

GTX106484 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
10% SDS PAGE
GTX106484 diluted at 1:1000

GTX106484_40135_IHC_19011506_668.jpg

GTX106484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using EIF2 beta(GTX106484) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106484_40135_IFA_19011506_698.jpg

GTX106484 ICC/IF Image

EIF2 beta antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF2S2 protein stained by EIF2 beta antibody (GTX106484) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX106484_40135_20150529_IHC_M_19011506_142.jpg

GTX106484 IHC-P Image

EIF2 beta antibody detects EIF2 beta protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
EIF2 beta antibody (GTX106484) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106484_40135_WB_M_liver_19011506_786.jpg

GTX106484 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX106484 diluted at 1:1000

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169