APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , Mouse liver , PC-12 , Rat2
Product Note
Knockdown/Knockout validation was supported by references (PMID:26990993)
Predict Reactivity
Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036838
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 2 subunit beta , EIF2 , EIF2B , EIF2beta , PPP1R67 , eIF-2-beta
Background
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106484 WB Image
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: Hela 10% SDS PAGE GTX106484 diluted at 1:1000
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GTX106484 IHC-P Image
EIF2 beta antibody detects EIF2 beta protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF2 beta antibody (GTX106484) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106484 WB Image
EIF2 beta antibody detects EIF2S2 protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE EIF2 beta antibody (GTX106484) dilution: 1:2000
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GTX106484 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SG xenograft, using EIF2 beta(GTX106484) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106484 WB Image
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE GTX106484 diluted at 1:1000
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GTX106484 ICC/IF Image
EIF2 beta antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2S2 protein stained by EIF2 beta antibody (GTX106484) diluted at 1:500. Blue: Hoechst 33343 staining.
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REFERENCE
REVIEW
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