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EIF2 beta antibody

KO/KD
Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484
Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106484
Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484
Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106484
Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484
Anti-EIF2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106484

Cat. No. GTX106484

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF2 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , Mouse liver , PC-12 , Rat2

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036838

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit beta , EIF2 , EIF2B , EIF2beta , PPP1R67 , eIF-2-beta

Background

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484

GTX106484 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
10% SDS PAGE
GTX106484 diluted at 1:1000

Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106484

GTX106484 IHC-P Image

EIF2 beta antibody detects EIF2 beta protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
EIF2 beta antibody (GTX106484) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484

GTX106484 WB Image

EIF2 beta antibody detects EIF2S2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
EIF2 beta antibody (GTX106484) dilution: 1:2000

Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106484

GTX106484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using EIF2 beta(GTX106484) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EIF2 beta antibody used in Western Blot (WB). GTX106484

GTX106484 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX106484 diluted at 1:1000

Anti-EIF2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106484

GTX106484 ICC/IF Image

EIF2 beta antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF2S2 protein stained by EIF2 beta antibody (GTX106484) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Hermosilla VE et al. Cell Death Dis 2024; 15 (3) : 223 Casein kinase 2 phosphorylates and induces the SALL2 tumor suppressor degradation in colon cancer cells.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
Knight JR et al. Biochem J 2015; 465 (2) : 227-38 Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.
Application : WB
Reactivity : Human
Rossi S et al. J Cell Sci 2015; 128 (9) : 1787-99 Nuclear accumulation of mRNAs underlies G4C2-repeat-induced translational repression in a cellular model of C9orf72 ALS.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169