GeneTex
United States (US)

EIF2 beta antibody

GTX115156_40534_IHC_18121410_231.jpg
GTX115156_40534_WB_18121410_233.jpg
GTX115156_40534_IFA_18121410_199.jpg

Cat No. GTX115156

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 2 Subunit Beta,Eif2,Eif2B,Eif2Beta,Ppp1R67,Eif-2-Beta,Eif2S2

Background

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]

Database

DATA IMAGES
GTX115156_40534_IHC_18121410_231.jpg

GTX115156 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF2 beta(GTX115156) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115156_40534_WB_18121410_233.jpg

GTX115156 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HeLa
10% SDS PAGE
GTX115156 diluted at 1:10000

GTX115156_40534_IFA_18121410_199.jpg

GTX115156 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF2 beta(GTX115156) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169