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EIF2 beta antibody

Anti-EIF2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115156
Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX115156
Anti-EIF2 beta antibody used in Western Blot (WB). GTX115156

Cat. No. GTX115156

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EIF2 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171341

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit beta , EIF2 , EIF2B , EIF2beta , PPP1R67 , eIF-2-beta

Background

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115156

GTX115156 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF2 beta(GTX115156) antibody at 1:500 dilution.

Anti-EIF2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX115156

GTX115156 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF2 beta(GTX115156) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF2 beta antibody used in Western Blot (WB). GTX115156

GTX115156 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HeLa
10% SDS PAGE
GTX115156 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169