*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , Raji
Bovine, Chimpanzee(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)
Recombinant protein encompassing a sequence within the center region of human EIF2B delta. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DKFZp586J0119 antibody, EIF-2B antibody, EIF2B antibody, EIF2Bdelta antibody, EIF2B4 antibody, translation initiation factor eIF-2b delta subunit antibody, translation initiation factor eIF-2B subunit delta antibody, eIF-2B GDP-GTP exchange factor subunit delta antibody, "eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa antibody"
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX115155 ICC/IF Image
EIF2B delta antibody detects EIF2B delta protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF2B delta protein stained by EIF2B delta antibody (GTX115155) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX115155 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115155 diluted at 1:1000