GeneTex
United States (US)

EIF2B delta antibody

GTX115155_40639_IFA_18121410_694.jpg
GTX115155_40639_WB_18121410_494.jpg

Cat No. GTX115155

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , Raji

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.61mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2B delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp586J0119 antibody, EIF-2B antibody, EIF2B antibody, EIF2Bdelta antibody, EIF2B4 antibody, translation initiation factor eIF-2b delta subunit antibody, translation initiation factor eIF-2B subunit delta antibody, eIF-2B GDP-GTP exchange factor subunit delta antibody, "eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa antibody"

Background

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX115155_40639_IFA_18121410_694.jpg

GTX115155 ICC/IF Image

EIF2B delta antibody detects EIF2B delta protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF2B delta protein stained by EIF2B delta antibody (GTX115155) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115155_40639_WB_18121410_494.jpg

GTX115155 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX115155 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169