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EIF2B epsilon antibody

Anti-EIF2B epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109799
Anti-EIF2B epsilon antibody used in Western Blot (WB). GTX109799
Anti-EIF2B epsilon antibody used in Western Blot (WB). GTX109799

Cat. No. GTX109799

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EIF2B epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse liver , H1299

Predict Reactivity

Rat, Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2B epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2B subunit epsilon , CACH , CLE , EIF-2B , EIF2Bepsilon , LVWM

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF2B epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109799

GTX109799 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using eIF2B epsilon(GTX109799) antibody at 1:200 dilution.

Anti-EIF2B epsilon antibody used in Western Blot (WB). GTX109799

GTX109799 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX109799 diluted at 1:1000

Anti-EIF2B epsilon antibody used in Western Blot (WB). GTX109799

GTX109799 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX109799 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169