GeneTex
United States (US)

EIF2B epsilon antibody

GTX30808_20170605_WB_18121410_469.jpg
GTX30808_20170605_ICCIF_18121410_678.jpg
GTX30808_20170605_ICCIF_1_18121410_214.jpg

Cat No. GTX30808

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
See all EIF2B epsilon products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1,000
ICC/IF 1:500-1:1,000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Rat1, HEK-293T, NIH3T3
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 50-65 of human eIF2ε conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 2B Subunit Epsilon,Cach,Cle,Eif-2B,Eif2Bepsilon,Lvwm,Eif2B5

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Database

DATA IMAGES
GTX30808_20170605_WB_18121410_469.jpg

GTX30808 WB Image

WB analysis of (1) 293T (2) HeLa (3) MCF-7 (4) A549 (5) A431 (6) 3T3 (7) C2 (8) Rat1 (9) PC-12 cells using GTX30808 eIF2B antibody at 1:500.
GTX30808_20170605_ICCIF_18121410_678.jpg

GTX30808 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX30808 EIF2B epsilon antibody at 1:1000 (red) with DAPI(blue).
GTX30808_20170605_ICCIF_1_18121410_214.jpg

GTX30808 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX30808 eIF2B antibody at 1:50(red) with Hoescht 33342 (blue) .
Package List Price ($)
$ 329