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EIF2B1 antibody

Anti-EIF2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109525
Anti-EIF2B1 antibody used in Western Blot (WB). GTX109525
Anti-EIF2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109525

Cat No. GTX109525

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human EIF2B1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164375

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2B subunit alpha , EIF2B , EIF2BA

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109525

GTX109525 ICC/IF Image

EIF2B1 antibody detects EIF2B1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF2B1 protein stained by EIF2B1 antibody (GTX109525) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-EIF2B1 antibody used in Western Blot (WB). GTX109525

GTX109525 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Jurkat
12% SDS PAGE
GTX109525 diluted at 1:10000

Anti-EIF2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109525

GTX109525 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF2B1(GTX109525) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169