*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 , H1299 , HeLa , HepG2
Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
2 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human EIF3 beta. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
eukaryotic translation initiation factor 3 subunit I , EIF3S2 , PRO2242 , TRIP-1 , TRIP1 , eIF3-beta , eIF3-p36
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
GTX101954 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using EIF3 beta(GTX101954) antibody at 1:200 dilution.
GTX101954 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101954 diluted at 1:1000