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EIF3 beta antibody [N2C3]

Anti-EIF3 beta antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101954
Anti-EIF3 beta antibody [N2C3] used in Western Blot (WB). GTX101954

Cat No. GTX101954

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit I , EIF3S2 , PRO2242 , TRIP-1 , TRIP1 , eIF3-beta , eIF3-p36

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Database

DATA IMAGES
Anti-EIF3 beta antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101954

GTX101954 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF3 beta(GTX101954) antibody at 1:200 dilution.

Anti-EIF3 beta antibody [N2C3] used in Western Blot (WB). GTX101954

GTX101954 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX101954 diluted at 1:1000

REFERENCE
There are currently no references for EIF3 beta antibody [N2C3] (GTX101954). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169