APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji
Predict Reactivity
Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human EIF3A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617867
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
eukaryotic translation initiation factor 3 subunit A , EIF3 , EIF3S10 , P167 , TIF32 , eIF3-p170 , eIF3-theta , p180 , p185
Cellular Localization
Cytoplasm
Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Database
Research Area
DATA IMAGES
![Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895](/templates/default/js/lightbox/images/loading.gif "Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895")
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GTX118895 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
GTX118895 diluted at 1:500
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GTX118895 ICC/IF Image
EIF3A antibody [N1N2], N-term detects EIF3A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3A protein stained by EIF3A antibody [N1N2], N-term (GTX118895) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX118895 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using EIF3A(GTX118895) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118895 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using EIF3A(GTX118895) antibody at 1:200 dilution.
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REFERENCE
REVIEW
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![Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895](/upload/website/prouct_img/small/GTX118895/GTX118895_40310_WB_w_23060519_429.webp "Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895](/upload/website/prouct_img/small/GTX118895/GTX118895_40310_20150410_IFA_w_23060519_895.webp "Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895](/upload/website/prouct_img/small/GTX118895/GTX118895_40310_IHC_w_23060519_180.webp "Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895](/upload/website/prouct_img/small/GTX118895/GTX118895_40310_IFA_w_23060519_554.webp "Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895](/upload/website/prouct_img/normal/GTX118895/GTX118895_40310_WB_w_23060519_429.webp "Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895](/upload/website/prouct_img/normal/GTX118895/GTX118895_40310_20150410_IFA_w_23060519_895.webp "Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895](/upload/website/prouct_img/normal/GTX118895/GTX118895_40310_IHC_w_23060519_180.webp "Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895")
![Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895](/upload/website/prouct_img/normal/GTX118895/GTX118895_40310_IFA_w_23060519_554.webp "Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895")