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EIF3D antibody

Anti-EIF3D antibody used in IHC (Paraffin sections) (IHC-P). GTX101424
Anti-EIF3D antibody used in Western Blot (WB). GTX101424
Anti-EIF3D antibody used in Western Blot (WB). GTX101424
Anti-EIF3D antibody used in IHC (Paraffin sections) (IHC-P). GTX101424
Anti-EIF3D antibody used in Western Blot (WB). GTX101424

Cat. No. GTX101424

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF3D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

HeLaS3 , mouse liver , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950187

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit D , EIF3S7 , eIF3-p66 , eIF3-zeta

Cellular Localization

Cytoplasm

Background

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF3D antibody used in IHC (Paraffin sections) (IHC-P). GTX101424

GTX101424 IHC-P Image

EIF3D antibody detects EIF3D protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
EIF3D antibody (GTX101424) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3D antibody used in Western Blot (WB). GTX101424

GTX101424 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101424 diluted at 1:5000

Anti-EIF3D antibody used in Western Blot (WB). GTX101424

GTX101424 WB Image

EIF3D antibody detects EIF3D protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
EIF3D antibody (GTX101424) dilution: 1:3000

Anti-EIF3D antibody used in IHC (Paraffin sections) (IHC-P). GTX101424

GTX101424 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HUH7 xenograft, using EIF3 zeta(GTX101424) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3D antibody used in Western Blot (WB). GTX101424

GTX101424 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101424 diluted at 1:1000

REFERENCE

Application Reference

Diep-Khanh Vo et al. Cancers (Basel) 2021; 13 (6) Interactome Mapping of eIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification.
Application : WB, ICC/IF
Reactivity : Human
Vo D.K. et al. Cancers 2021; 13 (6) : 1293 Interactome Mapping of eIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169