GeneTex
United States (US)

EIF3F antibody [N1C3]

GTX101967_39659_IHC_M_19011506_658.jpg
GTX101967_39659_IFA_19011506_596.jpg
GTX101967_40674_WB_19011506_464.jpg
GTX101967_39659_IHC_19011506_275.jpg

Cat No. GTX101967

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 3 Subunit F,Eif3S5,Eif3-P47,Eif3F

Cellular Localization

Cytoplasm

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Database

DATA IMAGES
GTX101967_39659_IHC_M_19011506_658.jpg

GTX101967 IHC-P Image

EIF3F antibody [N1C3] detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EIF3F antibody [N1C3] (GTX101967) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101967_39659_IFA_19011506_596.jpg

GTX101967 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF3F(GTX101967) antibody at 1:200 dilution.

GTX101967_40674_WB_19011506_464.jpg

GTX101967 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HepG2
10% SDS PAGE
GTX101967 diluted at 1:1000

GTX101967_39659_IHC_19011506_275.jpg

GTX101967 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF3F(GTX101967) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169