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EIF3F antibody [N1C3]

Anti-EIF3F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101967
Anti-EIF3F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101967
Anti-EIF3F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101967
Anti-EIF3F antibody [N1C3] used in Western Blot (WB). GTX101967

Cat. No. GTX101967

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EIF3F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240752

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit F , EIF3S5 , MRT67 , eIF3-p47

Cellular Localization

Cytoplasm

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Database

Research Area

DATA IMAGES

Anti-EIF3F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101967

GTX101967 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF3F(GTX101967) antibody at 1:200 dilution.

Anti-EIF3F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101967

GTX101967 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF3F(GTX101967) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101967

GTX101967 IHC-P Image

EIF3F antibody [N1C3] detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EIF3F antibody [N1C3] (GTX101967) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3F antibody [N1C3] used in Western Blot (WB). GTX101967

GTX101967 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HepG2
10% SDS PAGE
GTX101967 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169