*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , H1299 , HeLa , HepG2 , Molt-4 , Raji
Mouse, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
10 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Eukaryotic Translation Initiation Factor 3 Subunit K,Arg134,Eif3-P28,Eif3S12,Hspc029,M9,Mstp001,Plac-24,Plac24,Pro1474,Ptd001,Eif3K
Nucleus , Cytoplasm
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]
GTX101996 ICC/IF Image
EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3K protein stained by EIF3K antibody [N1C2] (GTX101996) diluted at 1:500.
Blue: Hoechst 33343 staining.
GTX101996 WB Image
Sample (30 ug of whole cell lysate)
12% SDS PAGE
GTX101996 diluted at 1:1000