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EIF3K antibody [N1C2]

Anti-EIF3K antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101996
Anti-EIF3K antibody [N1C2] used in Western Blot (WB). GTX101996

Cat No. GTX101996

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit K , ARG134 , EIF3-p28 , EIF3S12 , HSPC029 , M9 , MSTP001 , PLAC-24 , PLAC24 , PRO1474 , PTD001

Cellular Localization

Nucleus , Cytoplasm

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Database

DATA IMAGES
Anti-EIF3K antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101996

GTX101996 ICC/IF Image

EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3K protein stained by EIF3K antibody [N1C2] (GTX101996) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-EIF3K antibody [N1C2] used in Western Blot (WB). GTX101996

GTX101996 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX101996 diluted at 1:1000

REFERENCE
There are currently no references for EIF3K antibody [N1C2] (GTX101996). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169