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EIF3K antibody [N1C2]

Anti-EIF3K antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101996
Anti-EIF3K antibody [N1C2] used in Western Blot (WB). GTX101996

Cat. No. GTX101996

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EIF3K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240750

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit K , ARG134 , EIF3-p28 , EIF3S12 , HSPC029 , M9 , MSTP001 , PLAC-24 , PLAC24 , PRO1474 , PTD001

Cellular Localization

Nucleus , Cytoplasm

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-EIF3K antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101996

GTX101996 ICC/IF Image

EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3K protein stained by EIF3K antibody [N1C2] (GTX101996) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-EIF3K antibody [N1C2] used in Western Blot (WB). GTX101996

GTX101996 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX101996 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169