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EIF3K antibody

Anti-EIF3K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101995
Anti-EIF3K antibody used in IHC (Paraffin sections) (IHC-P). GTX101995
Anti-EIF3K antibody used in IHC (Paraffin sections) (IHC-P). GTX101995
Anti-EIF3K antibody used in Western Blot (WB). GTX101995

Cat No. GTX101995

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit K , ARG134 , EIF3-p28 , EIF3S12 , HSPC029 , M9 , MSTP001 , PLAC-24 , PLAC24 , PRO1474 , PTD001

Cellular Localization

Nucleus , Cytoplasm

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Database

DATA IMAGES
Anti-EIF3K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101995

GTX101995 ICC/IF Image

EIF3K antibody detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3K protein stained by EIF3K antibody (GTX101995) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-EIF3K antibody used in IHC (Paraffin sections) (IHC-P). GTX101995

GTX101995 IHC-P Image

EIF3K antibody detects EIF3K protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EIF3K antibody (GTX101995) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3K antibody used in IHC (Paraffin sections) (IHC-P). GTX101995

GTX101995 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF3K(GTX101995) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3K antibody used in Western Blot (WB). GTX101995

GTX101995 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX101995 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169