GeneTex
United States (US)

EIF3L antibody

IF3EI-antibody-GTX87704-WB-1_18121411_416.jpg

Cat No. GTX87704

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

IF3EI Antibody detects endogenous levels of total EIF3L protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human EIF3L.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 3 Subunit L,Eif3Eip,Eif3S11,Eif3S6Ip,Hspc021,Hspc025,Mstp005,Eif3L

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Database

DATA IMAGES
IF3EI-antibody-GTX87704-WB-1_18121411_416.jpg

GTX87704 WB Image

Western blot analysis of extracts from HuvEc cells, using IF3EI Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339