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EIF4A1 antibody [N2C2], Internal

Anti-EIF4A1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114910
Anti-EIF4A1 antibody [N2C2], Internal used in Western Blot (WB). GTX114910
Anti-EIF4A1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114910
Anti-EIF4A1 antibody [N2C2], Internal used in Western Blot (WB). GTX114910

Cat. No. GTX114910

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all EIF4A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620837

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4A1 , DDX2A , EIF-4A , EIF4A , eIF-4A-I , eIF4A-I

Database

Research Area

DATA IMAGES

Anti-EIF4A1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114910

GTX114910 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EIF4A1 antibody [N2C2], Internal (GTX114910) at 1:300 dilution.

Anti-EIF4A1 antibody [N2C2], Internal used in Western Blot (WB). GTX114910

GTX114910 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX114910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF4A1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114910

GTX114910 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1(GTX114910) antibody at 1:200 dilution.

Anti-EIF4A1 antibody [N2C2], Internal used in Western Blot (WB). GTX114910

GTX114910 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX114910 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169