GeneTex
United States (US)

EIF4A1 antibody [N2C2], Internal

GTX114910_WB_18121410_386.jpg
GTX114910_40240_IFA_18121410_912.jpg
GTX114910_40240_IHC_18121410_372.jpg

Cat No. GTX114910

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4A1,Ddx2A,Eif-4A,Eif4A,Eif-4A-I,Eif4A-I,Eif4A1

Database

DATA IMAGES
GTX114910_WB_18121410_386.jpg

GTX114910 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX114910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114910_40240_IFA_18121410_912.jpg

GTX114910 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1(GTX114910) antibody at 1:200 dilution.

GTX114910_40240_IHC_18121410_372.jpg

GTX114910 IHC-P Image

EIF4A1 antibody [N2C2], Internal detects EIF4A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EIF4A1 antibody [N2C2], Internal (GTX114910) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169