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EIF4A1 antibody

Anti-EIF4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107319
Anti-EIF4A1 antibody used in Western Blot (WB). GTX107319
Anti-EIF4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107319
Anti-EIF4A1 antibody used in Immunoprecipitation (IP). GTX107319

Cat No. GTX107319

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4A1,Ddx2A,Eif-4A,Eif4A,Eif-4A-I,Eif4A-I,Eif4A1

Database

DATA IMAGES
Anti-EIF4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107319

GTX107319 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF4A1(GTX107319) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4A1 antibody used in Western Blot (WB). GTX107319

GTX107319 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX107319 diluted at 1:3000

Anti-EIF4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107319

GTX107319 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1(GTX107319) antibody at 1:500 dilution.

Anti-EIF4A1 antibody used in Immunoprecipitation (IP). GTX107319

GTX107319 IP Image

Immunoprecipitation of EIF4A1 protein from HepG2 whole cell extracts using 5 μg of EIF4A1 antibody (GTX107319).
Western blot analysis was performed using EIF4A1 antibody (GTX107319).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169