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EIF4A1 antibody

Anti-EIF4A1 antibody used in Western Blot (WB). GTX107319
Anti-EIF4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107319
Anti-EIF4A1 antibody used in Immunoprecipitation (IP). GTX107319
Anti-EIF4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107319

Cat. No. GTX107319

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EIF4A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169036

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4A1 , DDX2A , EIF-4A , EIF4A , eIF-4A-I , eIF4A-I

Database

Research Area

DATA IMAGES

Anti-EIF4A1 antibody used in Western Blot (WB). GTX107319

GTX107319 WB Image

EIF4A1 antibody detects EIF4A1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A1 antibody (GTX107319) diluted by 1:3000.

Anti-EIF4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107319

GTX107319 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF4A1(GTX107319) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4A1 antibody used in Immunoprecipitation (IP). GTX107319

GTX107319 IP Image

Immunoprecipitation of EIF4A1 protein from HepG2 whole cell extracts using 5 μg of EIF4A1 antibody (GTX107319).
Western blot analysis was performed using EIF4A1 antibody (GTX107319).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-EIF4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107319

GTX107319 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1(GTX107319) antibody at 1:500 dilution.

REFERENCE

Application Reference

van Gastel J et al. Aging (Albany NY) 2019; 11 (23) : 11268-11313 The RXFP3 receptor is functionally associated with cellular responses to oxidative stress and DNA damage.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169