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EIF4A2 antibody

Anti-EIF4A2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101484
Anti-EIF4A2 antibody used in Western Blot (WB). GTX101484
Anti-EIF4A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101484
Anti-EIF4A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101484

Cat. No. GTX101484

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all EIF4A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa , HepG2

Product Note

Based on sequence homology, this antibody may cross react with EIF4A1 or EIF4A3 protein.

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4A2 , BM-010 , DDX2B , EIF4A , EIF4F , eIF-4A-II , eIF4A-II

Database

Research Area

DATA IMAGES

Anti-EIF4A2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101484

GTX101484 IHC-Wm Image

EIF4A2 antibody detects Eif4a2 protein at cytoplasm on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
EIF4A2 antibody (GTX101484) dilution: 1:200.
Anti-EIF4A2 antibody used in Western Blot (WB). GTX101484

GTX101484 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX101484 diluted at 1:10000

Anti-EIF4A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101484

GTX101484 IHC-P Image

EIF4A2 antibody detects EIF4A2 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
EIF4A2 antibody (GTX101484) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101484

GTX101484 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A2(GTX101484) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169