GeneTex
United States (US)

EIF4A2 antibody

GTX101485_WB_19011506_790.jpg

Cat No. GTX101485

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3

Specificity/Sensitivity

Based on sequence homology, this antibody may cross react with EIF4A1 or EIF4A3 protein.

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EIF4A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4A2,Bm-010,Ddx2B,Eif4A,Eif4F,Eif-4A-Ii,Eif4A-Ii,Eif4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Database

DATA IMAGES
GTX101485_WB_19011506_790.jpg

GTX101485 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX101485 diluted at 1:200

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169