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EIF4E2 antibody

Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524
Anti-EIF4E2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82524
Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524
Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524

Cat. No. GTX82524

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all EIF4E2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF Assay dependent
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11179164

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E family member 2 , 4E-LP , 4EHP , EIF4EL3 , IF4e , h4EHP

Database

Research Area

DATA IMAGES

Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524

GTX82524 WB Image

WB analysis of various samples using GTX82524 EIF4E2 antibody.
Lane 1: 293 whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: RD whole cell lysate
Lane 5: C2C12 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EIF4E2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82524

GTX82524 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX82524 EIF4E2 antibody.

Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524

GTX82524 WB Image

WB analysis of various samples using GTX82524 EIF4E2 antibody.
Lane 1: 293 whole cell lysate
Lane 2: RD whole cell lysate
Lane 3: HeLa whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: HL-60 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EIF4E2 antibody used in Western Blot (WB). GTX82524

GTX82524 WB Image

WB analysis of various samples using GTX82524 EIF4E2 antibody.
Lane 1: 293 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: RD whole cell lysate
Lane 5: NIH3T3 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

REFERENCE

Application Reference

JH Choi et al. Sci Adv 2024; 10 (29) : eadl5638 Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction
Evagelou SL et al. Mol Cell Biol 2020; 40 (6) DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation.
Sonia L. Evagelou et al. bioRxiv 2019; (Epub) DEAD-box protein family member DDX28 is a negative regulator of HIF-2α and eIF4E2-directed hypoxic translation.
Application : WB
Reactivity : Human
Frydryskova K et al. BMC Mol Biol 2016; 17 (1) : 21 Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules.
Application : ICC/IF
Reactivity : Human
Uniacke J et al. Nature 2012; 486 (7401) : 126-9 An oxygen-regulated switch in the protein synthesis machinery.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399