GeneTex
United States (US)

EIF4E3 antibody

EIF4E3-antibody-GTX86964-WB-1_18121410_770.jpg

Cat No. GTX86964

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

24 kDa. ( Note )

Specificity/Sensitivity

EIF4E3 Antibody detects endogenous levels of total EIF4E3 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human EIF4E3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4E Family Member 3,Eif-4E3,Eif4E-3,Eif4E3

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. May act as an inhibitor of EIF4E1 activity.

Database

Research Area

DATA IMAGES
EIF4E3-antibody-GTX86964-WB-1_18121410_770.jpg

GTX86964 WB Image

Western blot analysis of extracts from LOVO cells, using EIF4E3 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339