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EIF4G (phospho Ser1231) antibody

Orthogonal
Anti-EIF4G (phospho Ser1231) antibody used in IHC (Paraffin sections) (IHC-P). GTX79087
Anti-EIF4G (phospho Ser1231) antibody used in Western Blot (WB). GTX79087

Cat. No. GTX79087

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all EIF4G1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

175 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human eIF4G around the phosphorylation site of serine 1232 (P-V-Sp-P-L).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11169225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EIF4F,EIF4G,EIF4G1,EIF4GI,P220,PARK18,eukaryotic translation initiation factor 4 gamma 1

Background

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-EIF4G (phospho Ser1231) antibody used in IHC (Paraffin sections) (IHC-P). GTX79087

GTX79087 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX79087 EIF4G (phospho Ser1231) antibody.

Anti-EIF4G (phospho Ser1231) antibody used in Western Blot (WB). GTX79087

GTX79087 WB Image

Orthogonal

WB analysis of PMA treated 293 cell lysate using GTX79087 EIF4G (phospho Ser1231) antibody (Lane 3 and 4) and EIF4G antibody (Lane 1 and 2).

REFERENCE

Application Reference

Emma Mart?nez-Alonso et al. Int J Mol Sci 2022; 23 (3) Phosphorylation of Eukaryotic Initiation Factor 4G1 (eIF4G1) at Ser1147 Is Specific for eIF4G1 Bound to eIF4E in Delayed Neuronal Death after Ischemia.
Application : WB
Reactivity : Rat
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REVIEW

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Package List Price ($)
$ 399