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EIF4G3 antibody [N1], N-term

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Frozen sections) (IHC-Fr). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in Western Blot (WB). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in Immunoprecipitation (IP). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in Western Blot (WB). GTX118109
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

Cat. No. GTX118109

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF4G3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse testis

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4G3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167995

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4 gamma 3 , eIF-4G 3 , eIF4G 3 , eIF4GII

Database

Research Area

DATA IMAGES

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4G3 antibody [N1], N-term used in IHC (Frozen sections) (IHC-Fr). GTX118109

GTX118109 IHC-Fr Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EIF4G3 antibody [N1], N-term used in Western Blot (WB). GTX118109

GTX118109 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF4G3 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118109

GTX118109 ICC/IF Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF4G3 antibody [N1], N-term used in Immunoprecipitation (IP). GTX118109

GTX118109 IP Image

Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 μg of EIF4G3 antibody [N1], N-term (GTX118109).
Western blot analysis was performed using EIF4G3 antibody [N1], N-term (GTX118109).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-EIF4G3 antibody [N1], N-term used in Western Blot (WB). GTX118109

GTX118109 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:10000.

Anti-EIF4G3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX118109

GTX118109 IHC-P Image

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Bahat A et al. EMBO Mol Med 2024; 16 (3) : 523-546 Lowering mutant huntingtin by small molecules relieves Huntington's disease symptoms and progression.
Phaedra R Theodoridis et al. Proc Natl Acad Sci U S A 2021; 118 (7) Local translation in nuclear condensate amyloid bodies.
Application : WB
Reactivity : Human
M?ndez-Sol?s O et al. Cell Rep 2021; 37 (13) : 110144 Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis.
Application : WB
Reactivity : Human
Lin CJ et al. Small 2019; 15 (41) : e1902641 High Amplification of the Antiviral Activity of Curcumin through Transformation into Carbon Quantum Dots.
Application : WB
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169