GeneTex
United States (US)

EIF5 antibody

GTX114923_40401_WB_R_brain_18121410_705.jpg
GTX114923_40401_WB_18121410_877.jpg
GTX114923_40401_WB_M_brain_18121410_997.jpg
GTX114923_40401_IHC_18121410_548.jpg

Cat No. GTX114923

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

U87-MG , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 5,Eif-5,Eif-5A,Eif5

Background

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM]

Database

DATA IMAGES
GTX114923_40401_WB_R_brain_18121410_705.jpg

GTX114923 WB Image

EIF5 antibody detects EIF5 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
10% SDS-PAGE
EIF5 antibody (GTX114923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114923_40401_WB_18121410_877.jpg

GTX114923 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX114923 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114923_40401_WB_M_brain_18121410_997.jpg

GTX114923 WB Image

EIF5 antibody detects EIF5 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
10% SDS-PAGE
EIF5 antibody (GTX114923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114923_40401_IHC_18121410_548.jpg

GTX114923 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF5(GTX114923) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169