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EIF5 antibody

Anti-EIF5 antibody used in Western Blot (WB). GTX114923
Anti-EIF5 antibody used in Western Blot (WB). GTX114923
Anti-EIF5 antibody used in Western Blot (WB). GTX114923
Anti-EIF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114923

Cat. No. GTX114923

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

U87-MG , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 5 , EIF-5 , EIF-5A

Background

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-EIF5 antibody used in Western Blot (WB). GTX114923

GTX114923 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX114923 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF5 antibody used in Western Blot (WB). GTX114923

GTX114923 WB Image

EIF5 antibody detects EIF5 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
EIF5 antibody (GTX114923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF5 antibody used in Western Blot (WB). GTX114923

GTX114923 WB Image

EIF5 antibody detects EIF5 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10% SDS-PAGE
EIF5 antibody (GTX114923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114923

GTX114923 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF5(GTX114923) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sato K et al. EBioMedicine 2019; 44 : 387-402 Novel oncogene 5MP1 reprograms c-Myc translation initiation to drive malignant phenotypes in colorectal cancer.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169