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EIF5A antibody [AT2E9]

Anti-EIF5A antibody [AT2E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50006
Anti-EIF5A antibody [AT2E9] used in Western Blot (WB). GTX50006

Cat No. GTX50006

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2E9

Isotype

IgG1

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human EIF5A (1-154aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Eukaryotic Translation Initiation Factor 5A , Eif-5A , Eif5A1 , Eif5Ai , Eif5A

Cellular Localization

Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Background

EIF5A, previously known as EIF4D and IF-M2Ba, is a highly conserved 17-kDa protein that was originally characterized as a translation initiation factor, promoting the formation of the first peptide bond in mRNA translation in vitro. This is best positioned as a factor that controls the balance between cellular proliferation and death, depending upon the nature of cellular stress. Moreover, mutational analysis using human EIF5A to complement an EIF5A null strain of S. cerevisiae supports a role for EIF5A in protein synthesis. The activity of EIF5A is modulated by the covalent addition of the spermidine derivative hypusine to lysine residue 50.

Database

Research Area

DATA IMAGES

Anti-EIF5A antibody [AT2E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50006

GTX50006 ICC/IF Image

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human EIF5A antibody (1:500) with Alexa 488 (Green).

Anti-EIF5A antibody [AT2E9] used in Western Blot (WB). GTX50006

GTX50006 WB Image

The extracts of mouse lung and kidney (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EIF5A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319