In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human EIF5A (1-154aa) purified from E. coli
By protein-G affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Eukaryotic Translation Initiation Factor 5A , Eif-5A , Eif5A1 , Eif5Ai , Eif5A
Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
EIF5A, previously known as EIF4D and IF-M2Ba, is a highly conserved 17-kDa protein that was originally characterized as a translation initiation factor, promoting the formation of the first peptide bond in mRNA translation in vitro. This is best positioned as a factor that controls the balance between cellular proliferation and death, depending upon the nature of cellular stress. Moreover, mutational analysis using human EIF5A to complement an EIF5A null strain of S. cerevisiae supports a role for EIF5A in protein synthesis. The activity of EIF5A is modulated by the covalent addition of the spermidine derivative hypusine to lysine residue 50.