Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EIF5A antibody detects EIF5A protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EIF5A antibody (GTX111013) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EIF5A antibody detects EIF5A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF5A stained by EIF5A antibody (GTX111013) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EIF5A antibody detects EIF5A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
EIF5A antibody (GTX111013) dilution: 1:5000