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EIF6 antibody [HL1758]

Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408
Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408
Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408
Anti-EIF6 antibody [HL1758] used in IHC (Paraffin sections) (IHC-P). GTX637408

Cat No. GTX637408

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1758

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF6. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP

Cellular Localization

Cytoplasm,Nucleolus

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408

GTX637408 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (GTX637408) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408

GTX637408 WB Image

Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (GTX637408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [HL1758] used in Western Blot (WB). GTX637408

GTX637408 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (GTX637408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [HL1758] used in IHC (Paraffin sections) (IHC-P). GTX637408

GTX637408 IHC-P Image

EIF6 antibody [HL1758] detects EIF6 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
EIF6 stained by EIF6 antibody [HL1758] (GTX637408) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for EIF6 antibody [HL1758] (GTX637408). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EIF6 antibody [HL1758] (GTX637408). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations