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EIF6 antibody [N1C3-2]

KO/KD
Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971
Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971
Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971
Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971
Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971

Cat. No. GTX117971

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP

Cellular Localization

Cytoplasm , Nucleus

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

GTX117971 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

GTX117971 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

GTX117971 WB Image

Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

GTX117971 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971

GTX117971 IHC-P Image

EIF6 antibody [N1C3-2] detects EIF6 protein at cytoplasm and nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Machado HE et al. Nat Commun 2023; 14 (1) : 5092 Convergent somatic evolution commences in utero in a germline ribosomopathy.
Shengjiang Tan et al. Nat Commun 2021; 12 (1) : 5044 Somatic genetic rescue of a germline ribosome assembly defect.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169