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EIF6 antibody [N1C3-2]

Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971
Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

Cat No. GTX117971

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Drosophila, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173506

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP

Cellular Localization

Cytoplasm , Nucleus

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971

GTX117971 IHC-P Image

EIF6 antibody [N1C3-2] detects EIF6 protein at cytoplasm and nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971

GTX117971 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for EIF6 antibody [N1C3-2] (GTX117971). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EIF6 antibody [N1C3-2] (GTX117971). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169