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EIF6 antibody

Anti-EIF6 antibody used in Western Blot (WB). GTX54010
Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010
Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010
Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010

Cat No. GTX54010

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP

Cellular Localization

Cytoplasm,Nucleolus

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-EIF6 antibody used in Western Blot (WB). GTX54010

GTX54010 WB Image

WB analysis of various sample lysates using GTX54010 EIF6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010

GTX54010 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX54010 EIF6 antibody.
Blue : DAPI

Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010

GTX54010 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54010 EIF6 antibody.
Blue : DAPI
Dilution : 1:50

Anti-EIF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54010

GTX54010 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54010 EIF6 antibody.
Blue : DAPI
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339