ELAVL1 / HuR antibody [14F4]

Cat. No. GTX84577

Host	Mouse
Clonality	Monoclonal
Clone Name	14F4
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84577 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
IHC-P	1:50

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ELAVL1(NP_001410) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ELAV like RNA binding protein 1 , ELAV1 , HUR , Hua , MelG

Cellular Localization

Cytoplasm,Nucleus

Background

Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Database

Gene ID: 1994 ELAVL1
UniProt: Q15717 ELAVL1

Research Area

DATA IMAGES

GTX84577 WB Image

WB analysis of HEK293T cells transfected with ELAVL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84577 ELAVL1 antibody [14F4].
Loading : 5 ug per lane

GTX84577 IHC-P Image

IHC-P analysis of kidney tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of liver tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of prostate tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84577 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84577 ELAVL1 antibody [14F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

ELAVL1 / HuR antibody [14F4]

Cat. No. GTX84577

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84577 WB Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

GTX84577 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.