APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human HuR / ELAVL1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ELAV like RNA binding protein 1 , ELAV1 , ELAVL1 , HUR , Hua , MelG , HuR , HuA
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]
Database
Research Area
DATA IMAGES
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/templates/default/js/lightbox/images/loading.gif "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
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GTX00944 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ELAVL1 / HuR antibody [GT1182] (GTX00944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00944 WB Image
WB analysis of wild-type (WT) and ELAVL1 / HuR knockout (KO) 293T cell extracts using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Laoding : 25μg
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GTX00944 IHC-P Image
IHC-P analysis of mouse spleen tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100
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GTX00944 ICC/IF Image
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
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GTX00944 IP Image
IP analysis of 293T cell lysate using GTX00944 ELAVL1 / HuR antibody [GT1182].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000
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GTX00944 IHC-P Image
IHC-P analysis of human esophageal tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100
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GTX00944 WB Image
WB analysis of various samples using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Loading : 25μg
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GTX00944 IHC-P Image
IHC-P analysis of rat liver tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100
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GTX00944 ICC/IF Image
ICC/IF analysis of PFA-fixed U2OS cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
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GTX00944 ICC/IF Image
ICC/IF analysis of PFA-fixed C6 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
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REFERENCE
There are currently no references for ELAVL1 / HuR antibody [GT1182] (GTX00944). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ELAVL1 / HuR antibody [GT1182] (GTX00944). Be the first to share your experience with this product.
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_4000000101_20200313_WB_w_23053121_830.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_WB_1_w_23053121_709.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_IHC-P_9_w_23053121_858.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_ICC-IF_11_w_23053121_700.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunoprecipitation (IP). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20210323_IP_7_w_23053121_969.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunoprecipitation (IP). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_IHC-P_8_w_23053121_281.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_WB_2_w_23053121_667.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_IHC-P_7_w_23053121_889.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_ICC-IF_12_w_23053121_866.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/small/GTX00944/GTX00944_20200327_ICC-IF_10_w_23053121_559.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_4000000101_20200313_WB_w_23053121_830.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_WB_1_w_23053121_709.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_IHC-P_9_w_23053121_858.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_ICC-IF_11_w_23053121_700.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunoprecipitation (IP). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20210323_IP_7_w_23053121_969.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunoprecipitation (IP). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_IHC-P_8_w_23053121_281.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_WB_2_w_23053121_667.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Western Blot (WB). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_IHC-P_7_w_23053121_889.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in IHC (Paraffin sections) (IHC-P). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_ICC-IF_12_w_23053121_866.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")
![Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944](/upload/website/prouct_img/normal/GTX00944/GTX00944_20200327_ICC-IF_10_w_23053121_559.webp "Anti-ELAVL1 / HuR antibody [GT1182] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00944")