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ELK1 antibody [GT1257]

Recombinant antibody Orthogonal
Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854
Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854
Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854
Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854
Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

Cat. No. GTX02854

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1257

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all ELK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ELK1

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS transcription factor ELK1

Cellular Localization

Nucleus

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

GTX02854 WB Image

Orthogonal

HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

GTX02854 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

GTX02854 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

GTX02854 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ELK1 antibody [GT1257] used in Western Blot (WB). GTX02854

GTX02854 WB Image

WB analysis of various samples using GTX02854 ELK1 antibody [GT1257].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 419