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ELK1 antibody

Anti-ELK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50691
Anti-ELK1 antibody used in Western Blot (WB). GTX50691
Anti-ELK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50691

Cat No. GTX50691

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.387~391 (P-R-S-P-A) derived from human ELK1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS transcription factor ELK1

Cellular Localization

Nucleus

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ELK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50691

GTX50691 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50691 ELK1 antibody.

Anti-ELK1 antibody used in Western Blot (WB). GTX50691

GTX50691 WB Image

WB analysis of extracts from 293 and HT-29 cells using GTX50691 ELK1 antibody.

Anti-ELK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50691

GTX50691 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50691 ELK1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf