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ELK1 (phospho Ser383) antibody

Cat No. GTX11848

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
25 μl ($199)

APPLICATION

Application Note

WB: Use at a dilution of 1/1000. This concentration is determined using Elk1 fusion protein phosphorylated with purified Erk2. Low levels of endogenous Elk1 protein present in most cell types makes it difficult to detect by immunoblotting. Overexpression of Elk1 by transfection or immunoprecipitation, followed by immunoblotting will help improve detection. Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, PBS, 100μg/ml BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phospho-Ser383 peptide corresponding to residues around Ser383 (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS transcription factor ELK1

Cellular Localization

Nucleus

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 199