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ELK1 (phospho Ser383) antibody

Anti-ELK1 (phospho Ser383) antibody used in Western Blot (WB). GTX82701

Cat No. GTX82701

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Rat
Package
100 μl ($409)

APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS transcription factor ELK1

Cellular Localization

Nucleus

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ELK1 (phospho Ser383) antibody used in Western Blot (WB). GTX82701

GTX82701 WB Image

Western blot of non-phospho- and phospho-recombinant Elk-1 showing specific immunolabeling of the ~46 Elk-1 protein phosphorylated at Ser 383 using Elk-1 (phospho Ser383) antibody (GTX82701).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 409