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ELK1 (phospho Thr417) antibody

Anti-ELK1 (phospho Thr417) antibody used in IHC (Paraffin sections) (IHC-P). GTX53982
Anti-ELK1 (phospho Thr417) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53982

Cat No. GTX53982

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T417 of human ELK1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS transcription factor ELK1

Cellular Localization

Nucleus

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ELK1 (phospho Thr417) antibody used in IHC (Paraffin sections) (IHC-P). GTX53982

GTX53982 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX53982 ELK1 (phospho Thr417) antibody.

Anti-ELK1 (phospho Thr417) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53982

GTX53982 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX53982 ELK1 (phospho Thr417) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf