GeneTex
United States (US)

ELK3 antibody [N1C2]

GTX114966_40492_IHC_18121410_675.jpg
GTX114966_40492_IFA_18121410_947.jpg
GTX114966_40492_WB_18121410_385.jpg

Cat No. GTX114966

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ELK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Elk3, Ets Transcription Factor,Erp,Net,Sap-2,Sap2,Elk3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114966_40492_IHC_18121410_675.jpg

GTX114966 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ELK3(GTX114966) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114966_40492_IFA_18121410_947.jpg

GTX114966 ICC/IF Image

ELK3 antibody [N1C2] detects ELK3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ELK3 protein stained by ELK3 antibody [N1C2] (GTX114966) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114966_40492_WB_18121410_385.jpg

GTX114966 WB Image

Sample (30 µg of whole cell lysate)
A: NT2D1
B: IMR32
10% SDS PAGE
GTX114966 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf