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ELK3 antibody [N1C2]

Anti-ELK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114966
Anti-ELK3 antibody [N1C2] used in Western Blot (WB). GTX114966
Anti-ELK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114966

Cat. No. GTX114966

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ELK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

NT2D1 , IMR32

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ELK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177572

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS transcription factor ELK3 , ERP , NET , SAP-2 , SAP2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ELK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114966

GTX114966 ICC/IF Image

ELK3 antibody [N1C2] detects ELK3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ELK3 protein stained by ELK3 antibody [N1C2] (GTX114966) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ELK3 antibody [N1C2] used in Western Blot (WB). GTX114966

GTX114966 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: IMR32
10% SDS PAGE
GTX114966 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ELK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114966

GTX114966 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ELK3(GTX114966) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Keizo Takenaga et al. Int J Oncol 2021; 58 (3) : 397-408 Inhibition of the invasion and metastasis of mammary carcinoma cells by NBD peptide targeting S100A4 via the suppression of the Sp1/MMP?14 axis.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169