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ELK3 (phospho Ser357) antibody

Anti-ELK3 (phospho Ser357) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32324
Anti-ELK3 (phospho Ser357) antibody used in Western Blot (WB). GTX32324

Cat No. GTX32324

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($319)
See all ELK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of ELK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS transcription factor ELK3 , ERP , NET , SAP-2 , SAP2

Cellular Localization

Nucleus

Background

This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-ELK3 (phospho Ser357) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32324

GTX32324 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX32324 ELK3 (phospho Ser357) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-ELK3 (phospho Ser357) antibody used in Western Blot (WB). GTX32324

GTX32324 WB Image

WB analysis of PMA-treated HUVEC (A), PMA-treated HT29 (B) whole cell lysates using GTX32324 ELK3 (phospho Ser357) antibody.

REFERENCE

Application Reference

Kim KS et al. Sci Rep 2019; 9 (1) : 8418 ELK3 expressed in lymphatic endothelial cells promotes breast cancer progression and metastasis through exosomal miRNAs.
Application : WB
Reactivity : Human
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REVIEW

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Package List Price ($)
$ 319