GeneTex
United States (US)

ELK3 (phospho Ser357) antibody

GTX32324_20190131_ICCIF_19013102_596.jpg
GTX32324_20190131_WB_19013102_954.jpg

Cat No. GTX32324

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of ELK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Elk3, Ets Transcription Factor,Erp,Net,Sap-2,Sap2,Elk3

Cellular Localization

Nucleus

Background

This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES
GTX32324_20190131_ICCIF_19013102_596.jpg

GTX32324 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX32324 ELK3 (phospho Ser357) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32324_20190131_WB_19013102_954.jpg

GTX32324 WB Image

WB analysis of PMA-treated HUVEC (A), PMA-treated HT29 (B) whole cell lysates using GTX32324 ELK3 (phospho Ser357) antibody.