GeneTex
United States (US)

ELMO1 antibody [C3], C-term

GTX106301_WB_19011506_169.jpg
GTX106301_39701_WB_R_19011506_415.jpg
GTX106301_39701_IHC_19011506_453.jpg
GTX106301_39701_WB_M_19011506_138.jpg
GTX106301_39701_IFA_19011506_518.jpg

Cat No. GTX106301

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , *N2a , PC-12 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CED-12 antibody, CED12 antibody, ELMO-1 antibody, KIAA0281 antibody, MGC126406 antibody, ELMO1 antibody, engulfment and cell motility protein 1 antibody, ced-12 homolog 1 antibody, engulfment and cell motility 1 antibody

Cellular Localization

Cytoplasm , Cell membrane

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106301_WB_19011506_169.jpg

GTX106301 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
12% SDS PAGE
GTX106301 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106301_39701_WB_R_19011506_415.jpg

GTX106301 WB Image

ELMO1 antibody [C3], C-term detects ELMO1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
ELMO1 antibody [C3], C-term (GTX106301) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106301_39701_IHC_19011506_453.jpg

GTX106301 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach, using ELMO1(GTX106301) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106301_39701_WB_M_19011506_138.jpg

GTX106301 WB Image

ELMO1 antibody [C3], C-term detects ELMO1 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw 264.7 whole cell lysate/extract
G. 30 µg C2Cl2 whole cell lysate/extract
7.5% SDS-PAGE
ELMO1 antibody [C3], C-term (GTX106301) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106301_39701_IFA_19011506_518.jpg

GTX106301 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ELMO1(GTX106301) antibody at 1:500 dilution.

REVIEW

ELMO1 antibody [C3], C-term Cat No. GTX106301

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Amount used : 20μg
Blocking : 5% not-fat milk/PBS, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf